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Fig. 5 | Biological Research

Fig. 5

From: CtpB is a plasma membrane copper (I) transporting P-type ATPase of Mycobacterium tuberculosis

Fig. 5

Kinetic parameters of CtpB. The Cu+ ATPase activity of CtpB follows a Michaelis–Menten kinetics. Cu+ was added at different concentrations (0.25 to 10.0 μM) and the released phosphate from the hydrolysis of ATP was quantified in the enzymatic reactions. The kinetic parameters for CtpB embedded in the plasma membrane (KM 0.19 ± 0.04 μM and Vmax 2.29 ± 0.10 nmol/mg min) were calculated using the Prism v6.0 for Windows GraphPad Software, La Jolla California USA, http://www.graphpad.com. The plotted data correspond to the average activity ± SEM from three independent experiments

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