Fig. 3

LINC00460 regulated Raf1 by sponging miR-485-5p in PTC cells. a, b The predicted binding sites of LINC00460 and miR-485-5p, miR-485-5p and Raf1 3′-UTR. c, d Luciferase activity was examined in TPC1 and BCPAP cells cotransfected with LINC00460-WT or LINC00460-MUT and miR-485-5p mimics or miR-NC. e, f TPC1 and BCPAP cells were cotransfected with Raf1-WT or Raf1-MUT and miR-485-5p mimics or miR-NC, and luciferase activity was detected. g, h TPC1 and BCPAP cells were transfected with si-NC, si-LINC00460#1, si-LINC00460#2, pcDNA-LINC00460 (LINC00460) and pcDNA (Vector), respectively. The expression of miR-485-5p were detected by qRT-PCR after transfection. i, j The protein level of Raf1 was detected in TPC1 and BCPAP cells transfected with miR-NC, miR-485-5p mimics, anti-miR-NC and anti-miR-485-5p, respectively. k The expression of miR-485-5p in normal tissues and PTC tissues were examined by qRT-PCR. l The correlation between LINC00460 and miR-485-5p. m Raf1 expression in normal tissues and PTC tissues was detected by qRT-PCR. n The correlation between miR-485-5p and Raf1 in PTC tissues. **P < 0.01