Fig. 5

Overexpression of RASAL1 inhibited tumor growth in vivo. a HCT-116 cells were seeded into 6-well plate and transfected with vector control or 1 μg/well, 2 μg/well or 5 μg/well RASAL1 plasmid for 48 h, cell cycle related protein expression levels were detected using western blot. b HCT-116 cells were transfected with RASAL1 or control and injected subcutaneously into 12 nude mice in the flank region. Surgical resections of HCT-116 xenograft tumors on week 4 are shown. Measurements of tumor volumes were taken weekly. Data are expressed as mean ± SEM, *P  < 0.05. c Real-time PCR assay showed that RASAL1 expression was increased in the tumor tissues of RASAL1 overexpression group. Data are expressed as mean ± SEM, n = 4, *P < 0.05. d Western blotting assay showed that SCD1 expression was decreased in the tumor tissues of RASAL1 group. Data are expressed as mean ± SEM, n = 3, *P < 0.05