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Fig. 4 | Biological Research

Fig. 4

From: RASAL1 induces to downregulate the SCD1, leading to suppression of cell proliferation in colon cancer via LXRα/SREBP1c pathway

Fig. 4

RASAL1 inhibited the activity of SCD1 3′-UTR through LXRα and SREBP1c. a, b SCD1 mRNA and protein levels were detected by RT-qPCR and western blot at 48 h after transfection with pcDNA3.1 or SREBP1c, or treated with 1 μM T0901317, β-actin was used as loading control. c Luciferase assay of HCT-116 cells co-transfected with the luci-SCD1 3′-UTR reporter with SREBP1c or treated with 1 μM T0901317. After 48 h, firefly luciferase values were normalized to β-gal luciferase activity. d Schematic illustration of point mutation of the binding site of RASAL1 and SCD1 promoter. e, f The effect of RASAL1 on luciferase intensity controlled by the wild type (WT) or mutant (Mut) 3′-UTR of SCD1 was determined using the luciferase assay. Each bar represents the mean ± SEM. The results were reproduced in three independent experiments. *P < 0.05

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