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Fig. 2 | Biological Research

Fig. 2

From: RASAL1 induces to downregulate the SCD1, leading to suppression of cell proliferation in colon cancer via LXRα/SREBP1c pathway

Fig. 2

RASAL1 inhibited colon cancer cell proliferation accompanied by the inhibition of SCD1 expression. ad LoVo and HCT-116 cells were transfected with RASAL1 for 48 h. The CCK-8 assay showed that RASAL1 inhibited the proliferation of a LoVo and c HCT-116 cells. Absorbance at 450 nm was measured at 24, 48, and 72 h. Data are expressed as mean ± SEM, n = 3, *P < 0.05. Cell cycle of b LoVo and d HCT-116 were analyzed by PI staining. Data are expressed as mean ± SEM, n = 3, *P < 0.05. eh LoVo and HCT-116 cells were transfected with vector control and RASAL1 for 48 h, e, g followed by nile red staining, the lipid content was analyzed by flow cytometer, f, h RT-qPCR was used to detect the fatty acid synthesis related genes expression. i, j Western blotting and RT-qPCR assay were used to detect the SCD1 protein and mRNA expression levels in LoVo and HCT-116 cells transfected with RASAL1 for 48 h. Data are expressed as mean ± SEM, n = 3, *P < 0.05

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