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Fig. 5 | Biological Research

Fig. 5

From: FOXC2-AS1 regulates phenotypic transition, proliferation and migration of human great saphenous vein smooth muscle cells

Fig. 5

FOXC2-AS1 overexpression promotes phenotypic transition, proliferation, and migration of SV-SMCs through activating Notch pathway. a SV-SMCs were co-transfected with FOXC2-AS1 overexpression vector and FOXC2 siRNA (si-FOXC2). Western blot was performed to detect the protein levels of the Notch pathway-related proteins including Dll4, Notch1, Hey2 and EphrinB2. *p < 0.05 vs. Vector + si-Ctrl group. #p < 0.05 vs. si-Ctrl + FOXC2-AS1 group. SV-SMCs were transfected with FOXC2-AS1 overexpression vector and empty vector control, followed by treatment with Notch signaling pathway inhibitor FLI-06. b Western blot was performed to detect the protein levels of SM22α and OPN. c MTT assay was performed to detect cell proliferation, and d Transwell migration assay was performed to detect cell migration. Scale bar: 200 μm. *p < 0.05, **p < 0.01 vs. Vector group. #p < 0.05, ##p < 0.01 vs. FOXC2-AS1 group

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