Fig. 4
From: PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
Phenotypes and its transcription levels of TaPDIL1-2-silencing wheat plants. Grain starch contents and 1000-kernel weight of BSMV-TaPDIL1-2-silenced wheat plants, and transcription levels of TaAGPL1-1D gene. a Phenotypes of BSMV-TaPDIL1-2-, and BSVM-GFP-inoculated wheat spikes at 19 days after anthesis, respectively; b, c transcriptional levels of the TaPDIL1-2 gene at 19, 24, 29, and 34 days after anthesis detected by using the qPCR method with the wheat Actin (b) and GAPDH (c) genes as two internal controls; d and e, grain starch contents and 1000-kernel weight, respectively; f, g transcription levels of the TaAGPL1-1D genes in inoculated wheat grains at 19, 24, 29, and 34 days after anthesis with Actin (f) and GAPDH (g) genes as two internal controls, respectively. Each value is mean ± standard deviation of three biological replicates, and different letters indicate significant differences (P < 0.05) relative to control by using one-way ANOVA of Duncan’s multiple range test