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Fig. 2 | Biological Research

Fig. 2

From: PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

Fig. 2

Y1H assay between TaPDIL1-2 protein and TaAGPL1-1D promoter. a cDNA library screen of TaAGPL1-1D promoter in yeast cells; b different fragments (Pro-1, 1280 bp, Pro-2, 881 bp, and Pro-3, 494 bp) of TaAGPL1-1D promoter using Y1H assay; c yeast cells transformed with both the bait vectors containing the above three promoter fragments fused to a pAbAi reporter gene, and a prey vector containing TaPDIL1-2 fused to a pGADT7 activation domain. Yeast cells were grown in liquid media to an OD600 of 0.02, and diluted in a 10 × dilution series (from 100 to 10−3). Each dilution 5 μL was spotted on media selecting for plasmids (SD/-Leu, -Ura), supplemented with 0 and 100 ng/mL aureobasidin A (AbA)

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