Fig. 4

STAT3 is involved in ICAII/let-7i-mediated differentiation of ADSCs to SCs. a, b ADSCs were transfected with negative control mimic, let-7i mimic, let-7i mimic + pcDNA 3.0 vector, or let-7i mimic + STAT3 overexpression plasmid. qRT-PCR and western blot were performed to measure the expression level of STAT3 in ADSCs. c–e The expression levels of NGF, NT-3, S100, and P75 in ADSCs were determined by qRT-PCR and western blot. f Negative control mimic + pcDNA 3.0 vector, let-7i mimic + pcDNA 3.0 vector, or let-7i mimic + STAT3 overexpression plasmid were transfected into ADSCs after ICAII (10⁻⁷ mol/L) treatment. qRT-PCR was employed to detect the mRNA levels of NGF, NT-3, S100, and P75 in ADSCs. g, h The protein levels of NGF, NT-3, S100, and P75 in ADSCs were determined by western blot. *P < 0.05, ^#P < 0.05, ^&P < 0.05