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Fig. 4 | Biological Research

Fig. 4

From: IcarisideII facilitates the differentiation of ADSCs to SCs via let-7i/STAT3 axis to preserve erectile function

Fig. 4

STAT3 is involved in ICAII/let-7i-mediated differentiation of ADSCs to SCs. a, b ADSCs were transfected with negative control mimic, let-7i mimic, let-7i mimic + pcDNA 3.0 vector, or let-7i mimic + STAT3 overexpression plasmid. qRT-PCR and western blot were performed to measure the expression level of STAT3 in ADSCs. ce The expression levels of NGF, NT-3, S100, and P75 in ADSCs were determined by qRT-PCR and western blot. f Negative control mimic + pcDNA 3.0 vector, let-7i mimic + pcDNA 3.0 vector, or let-7i mimic + STAT3 overexpression plasmid were transfected into ADSCs after ICAII (10−7 mol/L) treatment. qRT-PCR was employed to detect the mRNA levels of NGF, NT-3, S100, and P75 in ADSCs. g, h The protein levels of NGF, NT-3, S100, and P75 in ADSCs were determined by western blot. *P < 0.05, #P < 0.05, &P < 0.05

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