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Fig. 3 | Biological Research

Fig. 3

From: IcarisideII facilitates the differentiation of ADSCs to SCs via let-7i/STAT3 axis to preserve erectile function

Fig. 3

STAT3 is a target gene of let-7i. a The binding sites between let-7i and STAT3 were predicted by Targetscan online database and the luciferase reporter plasmids containing the wild-type (WT) or mutated (MUT) STAT3 binding sites of let-7i were established. b The luciferase activity was measured in ADSCs co-transfected with STAT3-WT or STAT3-MUT luciferase reporter and let-7i mimic or negative control mimic c The luciferase activity was measured in ADSCs co-transfected with STAT3-WT or STAT3-MUT luciferase reporter and let-7i inhibitor or negative control inhibitor. d Western blot was conducted to measure protein level of STAT3 in ADSCs transfected with let-7i mimic, inhibitor, or their negative controls. e Western blot was performed to measure the protein level of STAT3 in ICAII (10−7 mol/L)-treated ADSCs. *P < 0.05

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