Fig. 8
From: Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
Apoptosis determination by tunnel assay. The cells were exposed to media with no additions (CONTROL), 28 µM E and its solvent control DMSO, 50 µM TMX, and 1.3 µM TMX and ethanol as their solvent control. This image represents two independent experiments. The first raw represents nuclei stained with DAPI, the second, the tunnel assay, and the third, the cells observed by phase contrast microscopy. The nuclei and phase contrast observations show that EP and TXM are reducing the number of cells in culture. Only 50 µM TMX gives a positive nuclear green fluorescent signal consistent with DNA fragmentation. Bar scale: 100 µm