Fig. 6
From: Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
MitoSOX mitochondrial oxygen reactive species assay. The assay was performed on 5000 cells exposed to 28 μM EP, 1 µM doxorubicin or DMSO control for 48 h. The cells were then exposed to mytoSOX and fluorescence was read at 510/595 nm excitation/emission. The results were normalized by the content of protein in each well, determined by sulforhodamine. The bars represent the mean values of the normalized fluorescence and standard error is shown