Fig. 5

COL1A1 is a target of miR-29b-3p. a The putative binding sites of COL1A1 and miR-29b-3p were predicted by TargetScan. b, c Luciferase activity was analyzed in HSF cells co-transfected with COL1A1-WT or COL1A1-MUT and miR-NC, miR-29b-3p, in-miR-NC or in-miR-29b-3p. d The expression of COL1A1 protein was measured in HSF cells transfected with miR-NC, miR-29b-3p, in-miR-NC or in-miR-29b-3p by western blot. e The association between the expression of COL1A1 and miR-29b-3p in burn wounds was analyzed. *P < 0.05