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Fig. 6 | Biological Research

Fig. 6

From: Neuroprotective effects of an Nrf2 agonist on high glucose-induced damage in HT22 cells

Fig. 6

IL-1β and IL-6 concentrations in different cell culture supernatants. HT22 cells were cultured in normal glucose medium (25 mM d-glucose), high glucose medium (50 mM d-glucose), high glucose medium + 1.25 μm/L SFN, high glucose medium + 2.5 μm/L SFN or high glucose medium + 5 μm/L  SFN for 72 h. IL-1β and IL-6 levels were measured using the Mouse IL-1β ELISA Kit and Mouse IL-6 ELISA Kit under a fluorescence micro-reader at a wavelength of 450 nm. The fluorescence intensity was correlated with IL-1β and IL-6 concentrations and the concentrations of IL-1β and IL-6 were calculated according the standard curve. *P < 0.05, **P < 0.01 vs. Normal Glucose Control Group; #P < 0.05, ##P < 0.01 vs. High Glucose Control Group

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