Fig. 1
Effect of PA-induced lipotoxicity on INS-1 cells. INS-1 cells were treated with various concentrations of PA for 24 h followed by MTT cell survival assay. Triplicate cell repeats were performed for each group. The absorbance at 570 nm was measured and showed a decrease in the percentage of cell survival with increasing doses of PA compared to the vehicle control. LD50 was calculated as 0.4 mM PA (indicated by the dotted lines). Values represent mean ± SEM and the data are representative of at least three independent experiments