Fig. 5

Overexpression and suppression of ORAI 1 affect the severity of adjuvant arthritis following resveratrol administration in rats model. 150 μl Freund’s complete adjuvant was injected into the left hind toe of male SD rats for 20 days to build AA model group while control groups were treated with 150 μl physiological saline in the same parts. All the animals were divided into 6 groups including control group, AA model group and other 4 drug intervention groups in which remaning model rats were respectively treated with 45 mg/kg, 45 mg/kg resveratrol + ORAI-1 overexpressive vector, ORAI-1 SiRNA and 200 mg/kg NAC for 12 days by intragastric administration and joint cavity microinjection with 10 rats/group. Polyarthritis index (b) and paw swelling degree (c) were recorded every 4 days during the drug-treating process. The Polyarthritis index was used to evaluate secondary lesions in each group of rats. Before the modeling procedure, the animal volume detector was applied to detect the affected hind paw volume of each rat (F0). From the onset of inflammation, the left hind paw volume was measured every 4 days (F1) and the paw swelling degree of the affected paw equal to F1/F0. All the mice were sacrificed in 12 days after treatment mentioned above. (a) The limbs were sectioned for histological studies. Whole blood samples were extracted via eye ball removal assay and serum were obtained via centrifugation and restored at − 80 °C. d–h Represent histogram demonstrate the serum level of IL-1, IL-6, IL-8, IL-10 and TNF-α were detected via ELISA assay. Results were presented as mean ± SD. All datasets were analyzed using one-way analysis of variance followed by Tukey’s post hoc test to compare the differential significance between each two groups. *P < 0.05 vs control and ^∆P < 0.05 vs Model group were considered statistically significant