Fig. 4

ORAI 1 overexpression restores resveratrol induced SOCE reduction following ATP or TG induction in FLSs. FLSs were respectively treated with 200 μM resveratrol single or coupled with ORAI1-vector within 5 μM H₂O₂. Both TG or ATP were utilized to induce calcium store depletion and the following adding of extracellular Ca²⁺ was used to trigger SOCE in FLSs. The real time fluctuation of [Ca²⁺]i was recorded via calcium imagine through confocal laszer stanning microscopy. Represented images (a) demonstrate the fluctuation of [Ca²⁺]i represent SOCE following ATP induced ER store depletion in three check point including before extracellular Ca²⁺ induction, reaching the peak level and becoming stable after induction. Summarized lincharts (b) and analysis (c) demonstrate the change of SOCE. Represented images (d) demonstrate the fluctuation of [Ca²⁺]i represent SOCE following TG induced ER store depletion in three check point including before extracellular Ca²⁺ induction, reaching the peak level and becoming stable after induction. Summarized lincharts (e) and analysis (f) demonstrate the change of SOCE. SOCE were calculated as F1(peak level of fluorescence)/F0(initial level of fluorescence). Results were presented as mean ± SD. All datasets were analyzed using one-way analysis of variance followed by Tukey’s post hoc test to compare the differential significance between each two groups. *P < 0.05 vs control was considered statistically significant