Fig. 3

ORAI 1 overexpression restores resveratrol induced apoptosis in FLSs. a FLSs were respectively treated with 200 μM Resveratrol, 200 μM Resveratrol + ORAI1-vector and ORAI1 SiRNA. Expressions and distribution of STIM1and ORAI1 were checked via immunofluorescence. ORAI1 and STIM1 were respectively labeled with green and red fluorescence via fluorescent secondary antibody of diverse species matched to primary antibody for STIM1 and ORAI1. DAPI were utilized to conduct nucleus dying with blue fluorescence. Merged pictures demonstrate the overlay among ORAI1, STIM1 and nucleus. b Apoptotic rate of FLSs receiving 200 μM Resveratrol, 200 μM Resveratrol + ORAI1-vector and ORAI1 SiRNA were checked via Annex V FITC/PI double staining method and detected via flow cytometry. The meanings of each quadrant are as follows: Lower left: normal cells; upper left: necrotic cells; lower right: early apoptotic cells; upper right: late apoptotic cells. c The proportion of apoptotic cells after corresponding treatment was illustrated as the combined percentage of both early and late apoptotic cells. Results were presented as mean ± SD and *P < 0.05 vs control, ^∆P < 0.05 vs 200 μM Res group was considered statistically significance. All datasets were analyzed using one-way analysis of variance followed by Tukey’s post hoc test to compare the differential significance between each two groups