Fig. 2

Resveratrol reduces ATP or TG induced SOCE via targeting ORAI1–STIM1 dimer in FLSs. FLSs were treated with diverse dose of resveratrol (50, 100, 200, 400 μM) within 5 μM H₂O₂. a STIM1 and ORAI1, two essential mediator for SOCE, were detected via western blotting. Represent histograms (c, d) demonstrate the analytical results of their expressive level following resveratrol treatment. ORAI1 SiRNA and ORAI1 overexpressed vector were respectively applied to knock down or upregulate ORAI1 expression. Co-IP was utilized to acess the inter-relationship between ORAI1 and STIM1. b FLSs were undergone diverse treatments including 200 μM resveratrol, 200 μm resveratrol + ORAI1 overexpressive vector and ORAI1 SiRNA and respectively subjected to immunoprecipitation with ORAI1 or STIM1 antibody. Western blot was applied to detect the expression of STIM1 and ORAI1 inside Co-IP (IP) or non-IP (WBL) samples. e–h Represent histograms demonstrate the exact analytical results.All datasets were analyzed using one-way analysis of variance followed by Tukey’s post hoc test to compare the differential significance between each two groups. Results were presented as mean ± SD and *P < 0.05 vs control, ^∆P < 0.05 vs 200 μM Res group was considered statistically significance