Fig. 1
Resveratrol reduces ATP or TG induced SOCE in FLSs. FLSs were treated with different dose of resveratrol under 5H2O2 and ATP or TG induced calcium depletion combined with subsequent SOCE induced via extracellular calcium were detected via calcium imaging. Fluo 8 AM was applied as a fluorescent probe for [Ca2+]i detected with green fluorescence. Represented line charts (b, d) and analysis (f, g) demonstrate the fluctuation of [Ca2+]i following ATP or TG induction. Represented images (a) demonstrate the fluctuation of [Ca2+]i represent SOCE in three check points including before extracellular calsium induction, reaching the peak level and becoming stable after extracellular calsium induction. Summarized line charts (c, e) and analysis (h, i) demonstrate the change of SOCE. Calcium depletion and SOCE were calculated as F1 (peak level of fluorescence)/F0 (initial level of fluorescence). Results were presented as mean ± SD. All datasets were analyzed using one-way analysis of variance followed by Tukey’s post hoc test to compare the differential significance between each two groups. *P < 0.05 vs control, ∆P < 0.05 vs 5H2O2 group was considered statistical significance