Fig. 5

Long-term effects of TFAP2C, GADD45B, and PMAIP1 expression levels on NSCLC cell proliferation. a The effects of TFAP2C overexpression, GADD45B knockdown, or PMAIP1 knockdown on cell proliferation of WI-26 VA4 cells were measured using colony-forming assays. b Quantitative analysis of the number of WI-26 VA4 cell clones after TFAP2C overexpression, GADD45B knockdown, or PMAIP1 knockdown was performed. The colonies of TFAP2C-overexpressing cells set at 100%. *p < 0.05 compared to cells treated with control vector or control siRNA. c The effects of TFAP2C overexpression, GADD45B knockdown, or PMAIP1 knockdown on WI-26 VA4 cell apoptosis were determined using caspase 3/7 assays. *p < 0.05 compared to cells treated with control vector or control siRNA. d The effects of TFAP2C, GADD45B, and/or PMAIP1 knockdown on cell proliferation of NCI-H292, NCI-H358, NCI-H460, or A549 cells were measured by using colony-forming assays. Representative images of each group are presented. e Quantitative analysis of the number of four NSCLC cell clones after the treatments was performed. The colonies of non-treated control cells set at 100%. *p < 0.05 compared to cells treated with control siRNA, **p < 0.05 compared to cells treated with TFAP2C siRNA. f The effects of TFAP2C, GADD45B, and/or PMAIP1 knockdown on apoptosis of NCI-H292, NCI-H358, NCI-H460, or A549 cells were measured by using caspase 3/7 assays. *p < 0.05 compared to cells treated with control siRNA, **p < 0.05 compared to cells treated with TFAP2C siRNA