Table 1 List of designed primers for real-time polymerase chain reaction
From: Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells
| Primer name | Primer sequence |
|---|---|
| Caspase3 (NM_004346.3) |
Forward: 5′ GTTTGAGCCTGAGCAGAGAC 3′ Reverse: 5′ TGGCAGCATCATCCACAC 3′ |
| Caspase-8 |
Forward: 5′ TGTGCCCAAATCAACAAGAG 3′ Reverse: 5′ TTCAAAGGTCGTGGTCAAAG 3′ |
| Ki67 |
Forward: 5′ GTGCGAAGGTTCTCATGC 3′ Reverse: 5′ CTTGACACTCCGCGTTAC 3′ |
| PCNA |
Forward: 5′ GCTCTTCCCTTACGCAAGTC 3′ Reverse: 5′ AGTGCCTCCAACACCTTC 3′ |
| RIPK3 |
Forward: 5′ CGCCTGCTGAAAGAAGTG 3′ Reverse: 5′ GTGAGCCTCCCTGAAATG 3′ |
| MLKL |
Forward: 5′ CCTGTTTCACCCATAAGC 3′ Reverse: 5′ GACTGCCTCAAAGTTTCC 3′ |
| GAPDH |
Forward: 5′ CACCCACTCCTCCACCTTTG 3′ Reverse: 5′ CCACCACCCTGTTGCTGTAG 3′ |