Fig. 6

BBR and DDP enhanced the expression and activation of RIPK3 and MLKL. OVCAR3 cells and POCCLs were treated with BBR (100 μM) and/or DDP (5 mg/L) for 24 h. The gross changes and necrotic morphology of OVCAR3 cells were detected by transmission electron microscopy (TEM) assay under ×4200 magnification (a). The expression levels of RIPK3 and MLKL in BBR and/or DDP-treated cells were detected by qRT-PCR (a, b) and Western blot (c, d). The phosphorylation of RIPK3 and MLKL was also detected by immunoblotting (c, d). Data were based on at least three independent experiments, and shown as mean ± SEM. *p < 0.05, **p < 0.01 compared with NC; ^#p < 0.05, ^##p < 0.01 compared with BBR; ^&p < 0.05, ^&&p < 0.01 compared with DDP