Fig. 6

Effects of Rop on the nucleus migration and the activity of caspase-3, the expression of apoptosis-related proteins in HCC cells. Bel 7402 cells (a) and HLE cells (b) were treated with Rop (2.0 mmol/L) for 24 h; the nucleus migration of caspase-3 in Bel 7402 cells and HLE cells were observed by laser confocal microscopy. The expression of caspase-3, activated caspase-3 (cleaved), PARP-1, activated PARP-1 (cleaved-PARP-1) and Bcl-2 (c, d). The expression of Apaf-1, caspase-9 (cleaved) and cytochrome C (e, f) in Bel 7402 cells and HLE cells were analysed by Western blotting. The bottom columnar graph depicts the statistical analysis of the relative expression of these proteins. The activity of caspase-3 in Bel 7402 cells (g) and HLE cells (h) was measured using an enzymatic reaction reagent kit. **P < 0.01 vs control group, N = 6. The images represent at least three independent experiments