Fig. 3

Influence of Rop on Bel 7402 cells and HLE cells apoptotic ratio. Bel 7402 cells (a) and HLE cells (b) were treated with the different concentrations (0.5 mmol/L, 1.0 mmol/L, 2.0 mmol/L) of Rop for 24 h. Trypan blue exclusion dye assay was used to analyse the apoptotic ratio of the cells. The images were observed by microscope, and the right columnar graph shows the statistical value of apoptotic ratio. *P < 0.05 and **P < 0.01 vs control group; N = 6. Bel 7402 cells (c) and HLE cells (d) were treated with 2 mmol/L of Rop for 48 h, and the apoptosis of Bel 7402 cells and HLE cells was analysed by flow cytometry. The right columnar graph shows the statistical analysis of the apoptosis ratios; *P < 0.05, **P < 0.01 vs. control groups. The images are a representation of at least three independent experiments