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Fig. 3 | Biological Research

Fig. 3

From: The long non-coding RNA H19 induces hypoxia/reoxygenation injury by up-regulating autophagy in the hepatoma carcinoma cells

Fig. 3

Knockdown of H19 inhibits the expression of pro-apoptotic factors and promotes anti-apoptotic factor in the Hep G2 cells. H19 siRNA was used as the knockdown of H19, 5 mM 3-MA in 24 h reoxygenation was used to inhibit autophagy. The expression of pro-apoptotic factors (cleaved caspase-3, -9 and Cyt c) and anti-apoptotic factor (Bcl-2) by western blot analysis. The intensity of each band was quantified by densitometry, and data were normalized to the β-actin signal. The expression levels in the control group were considered the basal levels, and the others are expressed as fold change from the control group. All data were from four independent experiments. **p < 0.01 vs. control group; #p < 0.05 vs. 8 h/24R group; ##p < 0.01 vs. 8 h/24R group

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