Fig. 2

Knockdown of H19 increases cell viability and decreases apoptotic rate in the Hep G2 cells. H19 siRNA was used as the knockdown of H19, 5 mM 3-MA in 24 h reoxygenation was used to inhibit autophagy. a Knockdown of H19 increases cell viability. Cell viability was measured by CCK-8 kit. The cells incubated with control medium were considered 100% viable. All data are mean ± S.E.M. of eight determinations. b Knockdown of H19 increases the cells in G0/G1 phase. The cells in G0/G1 phase were detected using propidium iodide methodology. c Detection of nuclear morphology in apoptotic cells by Hoechst 33342 staining. Apoptotic cells were identified as cells with condensed, disrupted nuclei (arrow, Hoechst staining, ×400). Scale bar 100 μm. Apoptotic cells in at least five random fields were counted. d Apoptosis analyzed by flow cytometry. All data were from four independent experiments. **p < 0.01 vs. control group; ^##p < 0.01 vs. 8 h/24R group