Fig. 1

The change of AST, ALT and LDH activities in the Hep G2 cells and the level of H19 mRNA in the Hep G2 and HCCLM3 cells. AST (a), ALT (b) and LDH (c) activities were detected in the cell culture fluid. Data are mean ± S.E.M. of eight determinations. The level of H19 mRNA in the Hep G2 (d) and HCCLM3 cells (e). The level of H19 mRNA was observed by real-time PCR analysis. The Ct of H19 from different groups in the Hep G2 cells: control: 22.50; 4 h/24R: 22.00; 8 h/24R: 19.00; 12 h/24R: 21.50. The Ct of H19 from different groups in the HCCLM3 cells: control: 22.00; 4 h/24R: 21.50; 8 h/24R: 19.50; 12 h/24R: 21.50. The Hep G2 cells in G0/G1 phase (f). The cells in G0/G1 phase were detected using propidium iodide methodology. The data were from four independent experiments. *p < 0.05 vs. control group; **p < 0.01 vs. control group