Fig. 2

On E14. TBX4-immunopositive cells were increased significantly in the epithelium of hindgut and URS, future anal orifice part of the cloacal membrane in normal group (a, b). The TBX4-immunopositive cells were scattered in the epithelium of the hindgut and dorsal cloacal membrane in ARM group (c, d). Red rectangles in a and c are shown at higher magnification in b and d. ×100 (c, d). ×400 (b, d). Black arrowheads: positive cells. H hindgut, U urogenital sinus, URS urorectal septum