Fig. 7
Assessment of PTEN/PI3K/AKT expression in NB4 cells after exposure to ATPR. a NB4 cells were pretreated with NAC for 1 h and then treated with ATPR (10−6 M) for 24 h. PI3K, AKT and P-AKT levels were analyzed by western blot. b NB4 cells were pretreated with NAC for 1 h and then treated with ATPR (10−6 M) for 72 h. b PTEN levels were analyzed by western blot. c A schematic of the proposed mechanism of ATPR-induced G0/G1 arrest and differentiation in human leukemia cells. The red arrows represent the inhibition of the subsequent signal, while the green arrows represent the promotion of the subsequent signal. *p < 0.05, **p < 0.01 versus control group; #p < 0.05, ##p < 0.01 versus ATPR group