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Fig. 6 | Biological Research

Fig. 6

From: ROS play an important role in ATPR inducing differentiation and inhibiting proliferation of leukemia cells by regulating the PTEN/PI3K/AKT signaling pathway

Fig. 6

ATPR induces the accumulation of intracellular ROS, leading to cell cycle arrest at G0/G1 and differentiation. a NB4 cells were treated with ATPR (10−6 M) for different durations (0–72 h). Intracellular ROS levels were assessed by flow cytometry. b Intracellular ROS levels were assessed by immunofluorescence staining after treatment with 10−6 M ATPR for 72 h. ce NB4 cells were pretreated with NAC and Tiron for 1 h and then treated with ATPR (10−6 M) for 72 h. The intracellular ROS levels were measured by flow cytometry, and CD11b and CD14 expression was measured by flow cytometry. f NB4 cells were pretreated with NAC for 1 h and then treated with ATPR (10−6 M) for 72 h. CD11b levels were analyzed by western blot. *p < 0.05, **p < 0.01 versus control group; #p < 0.05, ##p < 0.01 versus ATPR group

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