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Fig. 5 | Biological Research

Fig. 5

From: PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer

Fig. 5

MiR-365a-5p is frequently decreased in NSCLC and suppresses proliferation, migration and invasion of NSCLC cell lines. a The expression levels of miR-365a-5p in NSCLC cells (A549, SPCA1, H1299, H358, PC9) and human bronchial epithelial cells (16HBE) were detected by qRT-PCR. b The miR-365a-5p expression levels in 40 NSCLC tissues and 20 non-tumor tissues were detected by qRT-PCR. c Kaplan–Meier’s analysis of the correlation between miR-365a-5p expression and the overall survival rate of NSCLC patients. d The relative expression of miR-365a-5p was quantified by qRT-PCR in A549 and H1299 cells transfected with miR-365a-5p mimic (miR-365a-5p) or respective negative control (miR-NC). e, f MTT and colony formation assays showed overexpression of miR-365a-5p significantly suppressed proliferation of A549 and H1299 cells. g Wound-healing assays showed the repression of cell migration of A549 and H1299 due to overexpression of miR-365a-5p. h Transwell assays showed the repression of cell invasion of A549 and H1299 due to overexpression of miR-365a-5p. The data represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001

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