Fig. 4
From: Effects of glutamine deprivation on oxidative stress and cell survival in breast cell lines
Light microscopy images demonstrating morphology of MCF-10A cells after glutamine deprivation. Morphology of the MCF-10A cell line after 24 h, 48 h, 72 h and 96 h of glutamine deprivation compared to MCF-10A cells propagated in complete growth medium 24 h, 48 h, 72 h and 96 h. Glutamine deprivation for 72 h and 96 h decreased cell density slightly when compared to cells propagated in complete growth medium. Furthermore, MCF-10A cells appeared rounded following glutamine deprivation when compared to cells propagated in complete growth medium (×20 magnification)