Fig. 10

Glutamine deprivation results in the induction of apoptosis and necrosis. Graphs illustrating apoptosis induction after 24 h, 48 h, 72 h and 96 h of glutamine deprivation in MCF-7- (top left), MDA-MB-231- (top right), MCF-10A (bottom left) and BT-20 (bottom right) cell lines. Decreased cell viability was the most prominently observed following glutamine deprivation in the MCF-7 cell line (87.61% and 78.91%) after 24 h and 48 h. Viability in the MDA-MB-231 cell line decreased to 76% after 96 h of glutamine deprivation. The MCF10A- and BT-20 cell lines illustrated decreased cell viability to 94% and 91% respectively after 96 h of glutamine deprivation