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Table 1 List of designed primers for molecular studies

From: In vitro transplantation of spermatogonial stem cells isolated from human frozen–thawed testis tissue can induce spermatogenesis under 3-dimensional tissue culture conditions

Primer sequence Accession number Primer Cell types
Forward: 5′-GTACCTCTACCTGTGCTATGTG-3′
Reverse: 5′-TGTCATAGTCCTTCCTTCATCTC-3′
NM_001018011 PLZF Spermatogonia
Forward: 5′-CTGACAAGCAGCGGAACAAC-3′
Reverse: 5′-TCTTGGTCAAGGATGGCCTTT-3′
NM_053285.1 Tekt1 Spermatocyte
Forward: 5′-CAATCGCAATTACCGCTCCC-3′
Reverse: 5′-GGCTCCTCTCTGGCTTTGAT-3′
NM_003284.3 Tnp1 Spermatozoa
Forward: 5′-TCCCTGGAGAAGAGCTACG-3′
Reverse: 5′-GTAGTTTCGTGGATGCCACA-3′
NM_001101 β-actin Internal Control