Table 1 List of designed primers for molecular studies
Primer sequence | Accession number | Primer | Cell types |
|---|---|---|---|
Forward: 5′-GTACCTCTACCTGTGCTATGTG-3′ Reverse: 5′-TGTCATAGTCCTTCCTTCATCTC-3′ | NM_001018011 | PLZF | Spermatogonia |
Forward: 5′-CTGACAAGCAGCGGAACAAC-3′ Reverse: 5′-TCTTGGTCAAGGATGGCCTTT-3′ | NM_053285.1 | Tekt1 | Spermatocyte |
Forward: 5′-CAATCGCAATTACCGCTCCC-3′ Reverse: 5′-GGCTCCTCTCTGGCTTTGAT-3′ | NM_003284.3 | Tnp1 | Spermatozoa |
Forward: 5′-TCCCTGGAGAAGAGCTACG-3′ Reverse: 5′-GTAGTTTCGTGGATGCCACA-3′ | NM_001101 | β-actin | Internal Control |