Fig. 1

Notum inhibited Wnt 5a induced LX-2 cell activation. a 100 ng/ml Wnt 5a treatment enhanced LX-2 activation at a time-dependent manner, peaking at 36 h; *P < 0.05, ^ϮP < 0.05, ^ǂP < 0.05, ^※P < 0.05, compared with 0 h;, ^※P > 0.05, compared with ǂ. b–d pCMV6-AC-GFP-Notum was transfected into LX-2 cells. All cells were incubated with 100 ng/ml Wnt 5a for 72 h. b α-SMA, TIMP-1 and Col 1A1 mRNA expressions in LX-2 cells treated with Notum/Wnt 5a, *P < 0.05. c, d Protein expressions of α-SMA, TIMP-1, Col 1A1, NFATc1 and pJnk in LX-2 cells treated with Notum/Wnt 5a. The data are derived from three independent experiments