RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A

Biological Research

Table 1 Comparison of the editing sites detected in five mtDNA genes cotton materials

Gene	Position	Code	aa change	Editing efficiency (%)			Codon position
Gene	Position	Code	aa change	H276A	H276B	F1(H276A/H268)	Codon position
atp 1	1039	Ccc	P > S	100	100	100	1
	1064	tCg	S > L	100	100	100	2
	1216	cCt	L > F	100	100	100	2
	1292	cCg	P > L	100	100	100	2
	1415	cCa	P > L	100	100	100	2
	1484	cCa	P > L	100	100	100	2
atp4	115	Cgt	R > C	100	90.0	100	1
	212	tCg	S > L	83.3	100	100	2
	221	ttC	F > F	100	100	100	3
	224	cCc	P > L	83.3	100	100	2
	225	ccC	P > L	58.3	90.9	0	3
	245	cCt	P > L	100	100	91.7	2
	247	Ccg	P > L	50	27.2	100	1
	248	cCg	P > L	91.7	100	91.7	2
	392	tCa	S > L	83.3	100	100	2
	404	cCa	P > L	83.3	100	91.7	2
	413	aCt	T > I	83.3	100	91.7	2
	489	ccC	P > P	83.3	90.9	91.7	3
atp6	106	Cca	P > S	63.6	63.6	90.9	1
	242	cCg	P > L	54.5	45.5	63.6	2
	294	ttC	F > F	0	18.2	45.5	3
	305	tCg	S > L	45.5	54.5	72.7	2
	323	tCg	S > L	45.5	45.5	45.5	2
	331	Cgt	R > C	63.6	54.5	54.5	1
	338	cCc	P > L	36.4	54.5	63.6	2
	339	ccC	P > L	18.2	45.5	45.5	3
	733	Cat	H > Y	72.7	72.7	81.8	1
	740	tCt	S > F	45.5	63.6	72.7	2
	787	tCt	Q > *	63.6	45.5	81.8	1
atp 8	58	Ctc	L > F	100	84.6	100	1
	76	Cca	P > L	100	100	93.3	1
	77	cCa	P > L	100	100	93.3	2
	443	cCa	P > L	100	100	100	2
atp9	20	tCa	S > L	100	100	100	2
	50	tCa	S > L	85.7	93.3	100	2
	182	tCg	S > L	100	100	100	2
	191	cCa	P > L	92.6	100	100	2
	205	Ctg	L > L	85.7	86.7	92.9	1
	215	tCc	S > F	100	100	100	2
	223	Cga	R > *	100	100	100	1
	243	ttC	F > F	35.7	66.7	50	3

Position, respect to ATG. Code, capital word represents RNA editing site in code. All the editing forms are C-U conversion

ISSN: 0717-6287