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Table 1 Effect of cytokinins on indirect shoot morphogenesis from apical meristem-induced callus cultures of Gymnema sylvestre cultivated for 6 weeks on solid Murashige and Skoog medium

From: De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

Concentration of the cytokinins (mg L−1)
BAP TDZ Kin Number of shoots per ~ 0.5 g calli mass Shoot buds initiated (%) Length of the micro shoots in cm (mean ± SE)
0.5 3.94 ± 0.42d 41.16 ± 3.11c 1.34 ± 0.62d
1.0 6.87 ± 0.97a 63.42 ± 2.81a 2.17 ± 0.55b
2.0 7.92 ± 0.81a 79.42 ± 2.73a 2.38 ± 0.43a
3.0 5.39 ± 0.77b 58.4 ± 3.17b 2.24 ± 0.39a
4.0 4.03 ± 0.91c 49.81 ± 2.16c 2.14 ± 0.32b
  0.5 2.87 ± 0.34e 38.93 ± 2.18d 1.18 ± 0.48c
  1.0 5.43 ± 0.42b 58.37 ± 1.62b 2.01 ± 0.32b
  2.0 6.78 ± 0.67a 63.41 ± 2.44a 2.14 ± 0.41b
  3.0 4.93 ± 0.51c 51.22 ± 3.81b 2.02 ± 0.36b
  4.0 3.67 ± 0.47d 42.81 ± 2.94c 1.99 ± 0.29c
   0.5 2.14 ± 0.25e 31.42 ± 1.15d 1.02 ± 0.22e
   1.0 4.21 ± 0.33c 47.34 ± 1.26c 1.86 ± 0.34c
   2.0 5.17 ± 0.18b 54.49 ± 2.21b 2.02 ± 0.29b
   3.0 3.21 ± 0.26d 39.27 ± 2.45d 1.78 ± 0.41c
   4.0 2.11 ± 0.34e 31.38 ± 1.87d 1.56 ± 0.28d
  1. Obtained experimental data were inverse transformed prior to the statistical analysis. Mean values within column that are followed by the same letter are not significantly different at p < 0.05 levels
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