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Table 1 Effect of cytokinins on indirect shoot morphogenesis from apical meristem-induced callus cultures of Gymnema sylvestre cultivated for 6 weeks on solid Murashige and Skoog medium

From: De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

Concentration of the cytokinins (mg L−1)

BAP

TDZ

Kin

Number of shoots per ~ 0.5 g calli mass

Shoot buds initiated (%)

Length of the micro shoots in cm (mean ± SE)

0.5

3.94 ± 0.42d

41.16 ± 3.11c

1.34 ± 0.62d

1.0

6.87 ± 0.97a

63.42 ± 2.81a

2.17 ± 0.55b

2.0

7.92 ± 0.81a

79.42 ± 2.73a

2.38 ± 0.43a

3.0

5.39 ± 0.77b

58.4 ± 3.17b

2.24 ± 0.39a

4.0

4.03 ± 0.91c

49.81 ± 2.16c

2.14 ± 0.32b

 

0.5

2.87 ± 0.34e

38.93 ± 2.18d

1.18 ± 0.48c

 

1.0

5.43 ± 0.42b

58.37 ± 1.62b

2.01 ± 0.32b

 

2.0

6.78 ± 0.67a

63.41 ± 2.44a

2.14 ± 0.41b

 

3.0

4.93 ± 0.51c

51.22 ± 3.81b

2.02 ± 0.36b

 

4.0

3.67 ± 0.47d

42.81 ± 2.94c

1.99 ± 0.29c

  

0.5

2.14 ± 0.25e

31.42 ± 1.15d

1.02 ± 0.22e

  

1.0

4.21 ± 0.33c

47.34 ± 1.26c

1.86 ± 0.34c

  

2.0

5.17 ± 0.18b

54.49 ± 2.21b

2.02 ± 0.29b

  

3.0

3.21 ± 0.26d

39.27 ± 2.45d

1.78 ± 0.41c

  

4.0

2.11 ± 0.34e

31.38 ± 1.87d

1.56 ± 0.28d

  1. Obtained experimental data were inverse transformed prior to the statistical analysis. Mean values within column that are followed by the same letter are not significantly different at p < 0.05 levels