Skip to main content

Table 1 Biochemical characterization of by API20 E kit (bioMérieux, Inc.) and confirmed by tube assay

From: A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity

Biochemical test

PID15

B. gelatini (DSM 15865)

Characteristics

 Gram

+

+

 Temperature of growth (°C)

45–65

40–60

 pH growth range

6.0–11.0

4.0–10.0

Motility

+

+

 Aminoacid degradation

 l-Arginine dihydrolase

(−)

(−)

 l-Lysine decarboxylase

+

(−)

 l-Ornithine decarboxylase

+

(−)

 Tryptophan deaminase

(−)

(−)

 Indole

(−)

(−)

Metabolism

 Hugh-Leifson (oxidation)

+

+

 Hugh-Leifson (fermentation)

(−)

(−)

 Methyl red

(−)

(−)

 Catalase

+

+

 Oxidase

(−)

(−)

 Voges Proskauer

(−)

(−)

Hydrolysis of

 Gelatinase

+

+

 Starch

(−)

(−)

 β-galactosidase

(−)

(−)

Carbon source

 Citrate

(−)

(−)

 d-Glucose

+

V

 d-Mannitol

+

V

 Galactose

+

(−)

 Ribose

+

V

 d-Sucrose

+

(−)

 Lactose

+

(−)

Others

 H2S production

(−)

(−)

 Nitrate reduction

(−)

(−)

 Urease

(−)

(−)

  1. Strains: B. gelatini (DSM 15865) [22] and PID15 from the present study. Both microorganisms were tested under similar conditions. (+), positive; (−), negative; v, variable