Fig. 3

Immunogold labeling of tyrosinase, MITF, IL-4, IL-10, and TGF-β in artificial skin 10 days after treatment; Tyrosinase was thoroughly labeled in melanocytes and keratinocytes, especially on immature melanosomes (a), keratinocytic desmosomes and the cytoskeletal system (b), and cortical actin in the melanocyte dendritic tip (c), but not in mature melanin (d). Like tyrosinase, TGF-β (e–g) and MITF (h–j) were labeled on the cytoskeletal system and melanin was labeled in the melanocyte and keratinocyte. All sericin doses downregulated MITF and tyrosinase expression, upregulated TGF-β expression but did not affect expression of IL-4 and IL-10 (k)