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Fig. 4 | Biological Research

Fig. 4

From: MicroRNA-153-3p enhances cell radiosensitivity by targeting BCL2 in human glioma

Fig. 4

BCL2 was a downstream target of miR-153-3p in glioma cells. a The sequence alignment of human miR-153-3p with the 3′UTR of BCL2 is shown. The luciferase reporter constructs, BCL2 3′UTR-WT and BCL2 3′UTR-MUT were made using the seed sequence of miR-153-3p matching the 3′UTR of BCL2 mRNA. b HEK293 cells were co-transfected with the indicated plasmids and miR-153-3p or miR-NC for 48 h, and then they were subjected to the luciferase assay. U251 and U87 cells were transfected with miR-153-3p or miR-NC. The BCL2 mRNA (c) and protein (d) levels were determined by quantitative real-time PCR and Western blotting, respectively. Data represent mean ± SD. **p < 0.01 versus miR-NC; e the relative BCL2 mRNA levels in radioresistant glioma tumors (n = 20) and radiosensitive tumors (n = 25) were assessed using quantitative real-time PCR. ***p < 0.001 versus radiosensitive tumors; f correlation between the expression levels of BCL2 and miR-153-3p in radioresistant glioma tumors

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