Fig. 6

Drug tolerance assay. a HCT116 cells (LSD1 WT, +/+) stably expressing the USP38 protein and vector-transfected HCT116 cells (LSD1 WT, +/+) were treated with 40 ng/mL paclitaxel (PTX) for 4 days and CCK-8 was applied to measure cell proliferation. An asterisk (*) indicates p < 0.05 and double asterisks (**) indicate p < 0.01, compared to vector-transfected cells. b HCT116 cells (LSD1 WT, +/+) stably expressing the USP38 protein and vector-transfected HCT116 cells (LSD1 WT, +/+) were treated with 12.5 µg/mL 5-fluorouracil (5-FU) for 4 days and CCK-8 was applied to measure cell proliferation. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. c USP38 was expressed in SW48 cells (LSD1 WT, +/+), the cells were treated with PTX, and cell proliferation was analyzed. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. d USP38 was expressed in SW48 cells (LSD1 WT, +/+), the cells were treated with 5-FU, and cell proliferation was analyzed. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. e LSD1 single-allele knockout (KO, +/−) HCT116 cells were applied to be treated with PTX and cell proliferation was analyzed. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. f LSD1 single-allele knockout (KO, +/−) HCT116 cells were applied to be treated with 5-FU and cell proliferation was analyzed. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. Three independent experiments were performed