Fig. 3

USP38 enhances the activation of signaling pathways activated by LSD1. a The protein levels of LSD1 in wild-type (WT, +/+) and single-allele knockout (KO, +/−) HCT116 cells. Double asterisks (**) indicate p < 0.01, compared to wild-type cells. b After the Myc-tagged LSD1 gene was transfected into HEK293T cells, luciferase assays were performed to detect the activation of signaling pathways. In addition, after Myc-tagged LSD1 and/or Flag-tagged USP38 genes were transfected into HEK293T cells, luciferase assays were performed to detect the activation of signaling pathways as well. An asterisk (*) indicates p < 0.05 and double asterisks (**) indicate p < 0.01, compared to vector-transfected cells. Three independent experiments were performed