Fig. 1

The LSD1 protein is stabilized by the deubiquitinase USP38. a After tagged proteins of LSD1, JADE2 and USP38 proteins were expressed in HEK293T cells, the change in the LSD1 protein level was measured by western blotting. An asterisk (*) indicates p < 0.05, compared to LSD1-expressing or LSD1 and JADE2-expressing cells. b After LSD1 and USP38 or the USP38-C454A mutant were expressed in HEK293T cells, the change in the LSD1 protein level was measured by western blotting. An asterisk (*) indicates p < 0.05, compared to LSD1-expressing cells. c The Myc-tagged LSD1 gene was transfected into HEK293T cells together with the vector or Flag-tagged USP38 gene, and the cells were treated with cycloheximide (CHX) for 8 h. Western blotting was then performed to detect the changes in the protein level of Myc-LSD1. d The vector or Flag-tagged USP38 gene was transfected into HEK293T cells and the cells were treated with CHX for 8 h. Western blotting was then performed to detect the changes in the protein level of endogenously expressed LSD1. e Total RNAs were extracted from the vector- and Flag-USP38-transfected HEK293T cells and real-time quantitative PCR was performed to detect the changes in the LSD1 mRNA level. Three independent experiments were performed