Fig. 5
From: MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
Knockdown of SP1 inhibits cell proliferation and migration. a The expression of SP1 in HT29 cell lines with synthetic siRNAs transfection. b CCK-8 assay was performed to measure cell proliferation in HT29 cell line with synthetic siRNAs or miRNAs transfection. c Wound-healing assay was performed to measure cell migration in HT29 cell line with synthetic siRNAs or miRNAs transfection. d Western blot was conducted to measure the expression of E-Cadherin, N-Cadherin, Vimentin, and Snail. (***P < 0.001, **P < 0.01, ns: not significant) MIR-382: microRNA-382; NC: negative control; SP1: specific protein 1; si-SP1: siRNA targeting SP1; CCK-8: cell counting kit-8; OD 450: optical density at 450 nm