Table 1 Characteristics of 10 microsatellite loci and primer pair’s development for G. pumila
From: Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
Locus | Primer sequences (5′–3′) | Products size | Repeat motif | size (bp) | Dye | Ta (°C) | GenBank |
|---|---|---|---|---|---|---|---|
GP.7 | F: CGCATTCACTCACCCTCTCA | 309 | (ACTC)^4 | 360 | FAM | 52.1 | KX719822 |
R: TGGTTGGTGAAGGCTTTGGA | |||||||
GP.9 | F: ACCCGCTCTAGATCCTCTCT | 321 | (T)^10 | 378–380 | FAM | 60.5 | KX719823 |
R: AGGGGGAGTAATCAAGCCTCT | |||||||
GP.10 | F: GGGTACGGCGTAGTGGTAAT | 269 | (AT)^8 | 323–327 | VIC | 60.5 | KX719824 |
R: TCGTACAAAACCGCCCTCAA | |||||||
GP.12 | F: AGGATTATAGAGAGCCAGGTGGA | 138 | (CTT)^4 | 198–201 | NED | 52.1 | KX719825 |
R:CAGAAGACGAAATCGAAGCCG | |||||||
GP.13 | F: AGAGTAAGAGCTCTCTTCCGA | 248 | (ATT)^4 | 161–278 | NED | 52.1 | KX719826 |
R: GCAGACTCGAATCGGCAGTA | |||||||
GP.14 | F: GCATAGCCCGGTTGTCAAAC | 174 | (ACGC)^4 | 196–228 | PET | 52.1 | KX719827 |
R: ACCGAAAGATCCGACCATCG | |||||||
GP.15 | F: GGGCTGCTGCTCAATCAATT | 287 | (T)^10 | 347–351 | VIC | 52.1 | KX719828 |
R: ACCCGCTTCAAGTCATGATGA | |||||||
GP.16 | F: GCTATTTCTAGGGCCGGACC | 218 | (AT)^6 | 281–283 | FAM | 52.1 | KX719829 |
R: GCACAATACATAGATTCTGGATCGA | |||||||
GP.17 | F: GAGAGAAATCCACCAGGGCA | 145 | (T)^10 | 202–205 | VIC | 60.5 | KX719830 |
R: CAAGCGGACGACGTATACGA | |||||||
GP.18 | F: ACCGAAAGATCCGACCATCG | 174 | (GCGT)^4 | 192–228 | PET | 52.1 | KX719831 |
R: GCATAGCCCGGTTGTCAAAC |