Fig. 3

PMA pretreatment enhances cellular spreading of ADSCs. ADSCs pretreated with PMA (100 nM) for 4 h, and then the cells were seeded in a 4 well chamber slide (10³ cells/well) and allowed to attach and spread for 6 h. a Attached cells were fixed and Coomassie blue stained for clear visualization. After taking images of stained cells, the areas of individual cells were measured. The quantitative data were expressed as the mean ± S.E.M of at least 3 independent experiments. Scale bar = 200 μm, *p < 0.05. b F-actin of attached cells was stained with Texas Red-X phalloidin. Scale bar = 50 μm