Fig. 5
From: Thiamine deficiency contributes to synapse and neural circuit defects
Thiamine deficiency led to lowered excitatory neurotransmission, and impaired hippocampal long-term potentiation. a Brain slices showed significant GFP fluorescence in the CA1 region 3 weeks after injection with either control lentivirus (GFP only) or that expressing TPK RNAi. Arrows indicate electrophysiological recording sites. Scale bar represents 200 µm. b Input–out curves of fEPSP amplitude for mice injected with control (n = 11 cells numbers) or TPK RNAi (n = 14 cells numbers; P < 0.05 at 1.75 and P < 0.05 at 2.00) lentivirus. c LTP was impaired in neurons infected with TPK RNAi (n = 5 cells numbers), as compared with GFP control (n = 10 cells numbers). The inserts showed the raw records of the fEPSP before (a, b) and after (a’, b’) LTP induction by TBS stimulation. d Histogram showing that LTP could not be induced in TPK RNAi neurons. **P < 0.001 vs baseline (pre-TBS); #P < 0.01 vs GFP control by two-way ANOVA and post hoc tests. All the data are expressed as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.001, ***P < 0.0001, student’s t test and one-way ANOVA were used to determine the statistical significance of the differences