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Fig. 2 | Biological Research

Fig. 2

From: Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes

Fig. 2

MIR31HG knockdown inhibits HaCaT keratinocytes proliferation. a HaCaT keratinocytes were transfected with 50 nM scramble siRNA (siNC) or MIR31HG siRNA (siMIR31HG) for 48 h. qRT-PCR was performed to measure the expression of MIR31HG. Transcript levels were normalized to GAPDH expression. **P < 0.01. b CCK-8 assays were carried out to record the growth curves of HaCaT keratinocytes transfected with 50 nM siNC or siMIR31HG. **P < 0.01. c EdU incorporation assays were used to detect the proliferation rate of HaCaT keratinocytes transfected with 50 nM siNC or siMIR31HG for 48 h. EdU incorporation rate was analyzed using Image-pro Plus 6.0 software. Scale bars, 100 μm. **P < 0.01. d KRT6 and KRT16 expression was measured by qRT-PCR in HaCaT keratinocytes transfected with 50 nM siNC or siMIR31HG for 48 h. *P < 0.05. e The cell cycle distribution of HaCaT keratinocytes at 48 h post-transfection with 50 nM siNC or siMIR31HG was analyzed by flow cytometry. **P < 0.01

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