Fig. 4

Anti-miR-214 abolished the inhibitory effect of DKK3 knockdown on hypoxia-induced apoptosis in NRK-52E cells by inactivation of Wnt/β-catenin pathway. Western blot analysis of DKK3 level in kidney tissues of I/R-induced rat AKI models after 30 min of ischemia and 12 or 48 h of reperfusion (a) and cultured NRK-52E cells after hypoxia treatment (b). NRK-52E cells transfected with either si-DKK3 alone or combined with anti-miR-214 were incubated under hypoxia, followed by detection of DDK3 protein expression (c), apoptosis (d), caspase 3 activity (e), Bcl-2 and Bax protein levels (f), β-catenin, c-myc, cyclin D1 expression (g) mRNA expressions of TNF-α, IL-1β and IL-6 (h), and the concentrations of TNF-α, IL-1β and IL-6 (i). Each experiment was independently repeated 3 times. *P < 0.05